seurat

Version:

4.4

Category:

bio

Cluster:

Loki

Author / Distributor

https://satijalab.org/seurat/articles/get_started_v5_new

Description

Seurat (Satija Lab) is a unified, high-level framework for single-cell transcriptomics, multimodal data (e.g., RNA + ATAC), and spatial transcriptomics. It has a large user base and extensive documentation, making it an excellent starting point for new analysts.

Seurat v5 introduced a layered assay structure and improved support for large-scale datasets, including disk-backed matrices and sketch-based workflows for million-cell analyses. The ecosystem also supports reference mapping (e.g., Azimuth) and integrates well with multimodal workflows.

Documentation

[options]

Examples/Usage

  • List available modules:

    $ module avail seurat
    
  • Load the samtools module:

    $ module load
    
  • Check the loaded modules:

    $ module list
    
  • Unload the samtools module:

    $ module unload bio/SAMtools/1.15.1
    
  • create a sequence dictionary file:

    $ samtools dict
    
  • quickly check if SAM/BAM/CRAM file appears intact:

    $ samtools quickcheck
    

Installation

Source code is obtained from Seurat